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protein lobind tubes  (Eppendorf AG)


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    Structured Review

    Eppendorf AG protein lobind tubes
    Protein Lobind Tubes, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 201118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+lobind/Eppendorf/pmc13156593-183-28-31
    Average 99 stars, based on 201118 article reviews
    protein lobind tubes - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Peptide Fractionation:

    Article Title: Proteomic profiling of extracellular fluids to identify secreted proteins from muscle and fat tissues
    Article Snippet: .. TMTpro TM 18plex Isobaric Label Reagent Set (Proteome Sciences; Thermo Scientific, cat. no. A52045) High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific, cat. no. 84868) pH paper (Thermo Fisher, cat. no. 13-640-508) Empore, solid phase extraction disk, C18 (CDS, cat. no. 2215-C18) 15 mL Conical Centrifuge Tubes (Thermo Fisher, cat. no. 1495953A) Microcentrifuge tubes, 2 mL Protein LoBind (Eppendorf, cat. no. 022431102) Axygen deep well plate (P-DW-11-C-S) Autosampler plastic or glass vial insert (Agilent, cat. no. 5181–8872) Glass vial, crimp top, 2 mL (Agilent, cat. no. 5151–3375) Crimp cap, 11 mm (Agilent, cat. no. 5151–1211) C18 SEPPAK column (Waters, WAT054955 or WAT023590) 5 μm Zorbax 300 Extended-C18, 4.6 × 250mm (Agilent, 770995–902) Vacuum manifold (e.g. Thermo Fisher, cat. no. TF60104–233) Vial crimper, 11 mm, manual (Agilent, cat. no. 5040–4667) Vacuum centrifuge (e.g. LABCONCO, CentriVap System) Vortex Mixer (e.g. Scientific Industry, Vortex-Genie 2) Heating Block (e.g. .. Benchmark, Multi-Therm Vortex Shaker) Centrifuge, refrigerated (e.g. Thermo Scientific, Sorvall Legend X1R) HPLC (e.g. Agilent, 1200 HPLC) HPLC C18 column (e.g. Agilent 300Extend, 3.5 μm particles, 4.6 mm ID, 220 mm long) 5 M NaCl: dissolve 29.22 g in 100 mL LC-MS H 2 O 1 M HEPES: dissolve 23.83 g in 100 mL LC-MS H 2 O and adjust pH to 8.5–8.8 using NaOH, filter, store at 4 °C 1 M DTT: dissolve 38.6 mg in 250 μL LC-MS H 2 O 0.5 M IAA: dissolve 92.5 mg in 1 mL LC-MS H 2 O 200 mM EPPS buffer, pH 8.5: dissolve 5.0466 g in 100 mL LC-MS H 2 O and adjust pH to 8.5 using NaOH 5% Hydroxylamine: dilute 50% Hydroxylamine 1:10 in LC-MS H 2 O 70% ACN/1%FA: for 100 mL combine 29 mL LC-MS H 2 O, 70 mL ACN, and 1 mL FA.

    Extraction:

    Article Title: Proteomic profiling of extracellular fluids to identify secreted proteins from muscle and fat tissues
    Article Snippet: .. TMTpro TM 18plex Isobaric Label Reagent Set (Proteome Sciences; Thermo Scientific, cat. no. A52045) High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific, cat. no. 84868) pH paper (Thermo Fisher, cat. no. 13-640-508) Empore, solid phase extraction disk, C18 (CDS, cat. no. 2215-C18) 15 mL Conical Centrifuge Tubes (Thermo Fisher, cat. no. 1495953A) Microcentrifuge tubes, 2 mL Protein LoBind (Eppendorf, cat. no. 022431102) Axygen deep well plate (P-DW-11-C-S) Autosampler plastic or glass vial insert (Agilent, cat. no. 5181–8872) Glass vial, crimp top, 2 mL (Agilent, cat. no. 5151–3375) Crimp cap, 11 mm (Agilent, cat. no. 5151–1211) C18 SEPPAK column (Waters, WAT054955 or WAT023590) 5 μm Zorbax 300 Extended-C18, 4.6 × 250mm (Agilent, 770995–902) Vacuum manifold (e.g. Thermo Fisher, cat. no. TF60104–233) Vial crimper, 11 mm, manual (Agilent, cat. no. 5040–4667) Vacuum centrifuge (e.g. LABCONCO, CentriVap System) Vortex Mixer (e.g. Scientific Industry, Vortex-Genie 2) Heating Block (e.g. .. Benchmark, Multi-Therm Vortex Shaker) Centrifuge, refrigerated (e.g. Thermo Scientific, Sorvall Legend X1R) HPLC (e.g. Agilent, 1200 HPLC) HPLC C18 column (e.g. Agilent 300Extend, 3.5 μm particles, 4.6 mm ID, 220 mm long) 5 M NaCl: dissolve 29.22 g in 100 mL LC-MS H 2 O 1 M HEPES: dissolve 23.83 g in 100 mL LC-MS H 2 O and adjust pH to 8.5–8.8 using NaOH, filter, store at 4 °C 1 M DTT: dissolve 38.6 mg in 250 μL LC-MS H 2 O 0.5 M IAA: dissolve 92.5 mg in 1 mL LC-MS H 2 O 200 mM EPPS buffer, pH 8.5: dissolve 5.0466 g in 100 mL LC-MS H 2 O and adjust pH to 8.5 using NaOH 5% Hydroxylamine: dilute 50% Hydroxylamine 1:10 in LC-MS H 2 O 70% ACN/1%FA: for 100 mL combine 29 mL LC-MS H 2 O, 70 mL ACN, and 1 mL FA.

    Blocking Assay:

    Article Title: Proteomic profiling of extracellular fluids to identify secreted proteins from muscle and fat tissues
    Article Snippet: .. TMTpro TM 18plex Isobaric Label Reagent Set (Proteome Sciences; Thermo Scientific, cat. no. A52045) High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific, cat. no. 84868) pH paper (Thermo Fisher, cat. no. 13-640-508) Empore, solid phase extraction disk, C18 (CDS, cat. no. 2215-C18) 15 mL Conical Centrifuge Tubes (Thermo Fisher, cat. no. 1495953A) Microcentrifuge tubes, 2 mL Protein LoBind (Eppendorf, cat. no. 022431102) Axygen deep well plate (P-DW-11-C-S) Autosampler plastic or glass vial insert (Agilent, cat. no. 5181–8872) Glass vial, crimp top, 2 mL (Agilent, cat. no. 5151–3375) Crimp cap, 11 mm (Agilent, cat. no. 5151–1211) C18 SEPPAK column (Waters, WAT054955 or WAT023590) 5 μm Zorbax 300 Extended-C18, 4.6 × 250mm (Agilent, 770995–902) Vacuum manifold (e.g. Thermo Fisher, cat. no. TF60104–233) Vial crimper, 11 mm, manual (Agilent, cat. no. 5040–4667) Vacuum centrifuge (e.g. LABCONCO, CentriVap System) Vortex Mixer (e.g. Scientific Industry, Vortex-Genie 2) Heating Block (e.g. .. Benchmark, Multi-Therm Vortex Shaker) Centrifuge, refrigerated (e.g. Thermo Scientific, Sorvall Legend X1R) HPLC (e.g. Agilent, 1200 HPLC) HPLC C18 column (e.g. Agilent 300Extend, 3.5 μm particles, 4.6 mm ID, 220 mm long) 5 M NaCl: dissolve 29.22 g in 100 mL LC-MS H 2 O 1 M HEPES: dissolve 23.83 g in 100 mL LC-MS H 2 O and adjust pH to 8.5–8.8 using NaOH, filter, store at 4 °C 1 M DTT: dissolve 38.6 mg in 250 μL LC-MS H 2 O 0.5 M IAA: dissolve 92.5 mg in 1 mL LC-MS H 2 O 200 mM EPPS buffer, pH 8.5: dissolve 5.0466 g in 100 mL LC-MS H 2 O and adjust pH to 8.5 using NaOH 5% Hydroxylamine: dilute 50% Hydroxylamine 1:10 in LC-MS H 2 O 70% ACN/1%FA: for 100 mL combine 29 mL LC-MS H 2 O, 70 mL ACN, and 1 mL FA.

    other:

    Article Title: Utilizing Constrained Bicyclic Peptides for In Vitro Diagnostics
    Article Snippet: Gold nanoparticle and platinum nanocatalyst solutions were handled and stored in Protein LoBind sample tubes (Eppendorf) or glass sample vials.

    Organoids:

    Article Title: Rank signaling drives basal cell-lineage infidelity leading to mammary tumorigenesis.
    Article Snippet: Organoids were isolated by sequential treatments with Red Blood Cell Lysing Buffer Hybri-Max (Sigma-Aldrich, #R7757-100ML), for 1 min at RT, 20U/ml DNAseI/ 0.5mg/ml AR TI CL E IN P RE SS dispase II (Roche, #10104159001 / ThermoFisher Scientific, #17105041) at 37°C for 5 min, and 5 rounds of differential centrifugations (450g for 10 sec). .. Pelleted organoids were collected in Protein LoBind 1.5ml tubes (Eppendorf, #022431081) and snap-frozen at -80oC before lysis for proteomic analysis. ..

    Lysis:

    Article Title: Rank signaling drives basal cell-lineage infidelity leading to mammary tumorigenesis.
    Article Snippet: Organoids were isolated by sequential treatments with Red Blood Cell Lysing Buffer Hybri-Max (Sigma-Aldrich, #R7757-100ML), for 1 min at RT, 20U/ml DNAseI/ 0.5mg/ml AR TI CL E IN P RE SS dispase II (Roche, #10104159001 / ThermoFisher Scientific, #17105041) at 37°C for 5 min, and 5 rounds of differential centrifugations (450g for 10 sec). .. Pelleted organoids were collected in Protein LoBind 1.5ml tubes (Eppendorf, #022431081) and snap-frozen at -80oC before lysis for proteomic analysis. ..

    Analysis:

    Article Title: Rank signaling drives basal cell-lineage infidelity leading to mammary tumorigenesis.
    Article Snippet: Organoids were isolated by sequential treatments with Red Blood Cell Lysing Buffer Hybri-Max (Sigma-Aldrich, #R7757-100ML), for 1 min at RT, 20U/ml DNAseI/ 0.5mg/ml AR TI CL E IN P RE SS dispase II (Roche, #10104159001 / ThermoFisher Scientific, #17105041) at 37°C for 5 min, and 5 rounds of differential centrifugations (450g for 10 sec). .. Pelleted organoids were collected in Protein LoBind 1.5ml tubes (Eppendorf, #022431081) and snap-frozen at -80oC before lysis for proteomic analysis. ..



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